High-purity his-tagged protein purification with TALON resin
TALON resin for his-tagged protein purification lets you prepare exceptionally pure his-tagged proteins from bacterial, mammalian, yeast, and baculovirus-infected cells, under native or denaturing conditions. TALON resin is an immobilized metal affinity chromatography (IMAC) resin charged with cobalt, which binds to his-tagged proteins with higher specificity than nickel-charged resins. As a result, TALON resin delivers his-tagged proteins of the highest purity. In addition, each cobalt ion is bound to the resin at four sites, resulting in low metal ion leakage. TALON resin is available in a variety of formats. Use the following tables to decide which one is best for your specific application.
Prepacked formats | |||
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HisTALON Superflow Cartridge | HisTALON Gravity Columns | TALON Spin Columns | |
Prepacked columns | Yes | Yes | Yes |
Binding capacity (mg protein/ml resin) | 5–20 | 5–20 | 4–8 |
Starting material | Clarified lysate | Clarified lysate | Clarified lysate |
FPLC applications | Yes | No | No |
Time savings | +++ | +++ | +++++ |
Maximum pressure |
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Maximum flow rate (ml/min) |
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N/A | N/A |
Bead size (µm) | 60–160 | 45–165 | 45–165 |
Downstream applications |
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Bulk resin formats |
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TALON Metal Affinity Resin | TALON Superflow Metal Affinity Resin | TALON Magnetic Beads | TALON CellThru | |
Prepacked columns | No | No | No | No |
Binding capacity (mg protein/ml resin) | 5–15 | 5–18 | 2–15 | 5–10 |
Starting material | Clarified lysate | Clarified lysate | Clarified lysate | Unclarified lysate |
FPLC applications | No | Yes | No | No |
Time savings | + | + | +++ | +++ |
Maximum pressure |
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Maximum flow rate (ml/min) | 0.5 | 50 | N/A | 13 |
Bead size (µm) | 45–165 | 60–160 | 20–75 | 300–500 |
Downstream applications |
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Why choose TALON resin?
Obtain highest purity with cobalt resin
Prepare exceptionally pure his-tagged proteins from bacterial, mammalian, yeast, and baculovirus-infected cells.
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